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Phase Separation of Membrane Scaffolding Proteins as a Mechanism to Control Formation of Tight Junctions
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Phase Separation of Membrane Scaffolding Proteins as a Mechanism to Control Formation of Tight Junctions
Phase Separation of Membrane Scaffolding Proteins as a Mechanism to Control Formation of Tight Junctions
In this project we aim to understand how tight junctions (TJ) assemble into a continuous sub-apical belt in epithelial cells. Based on cell biological and in vitro data we hypothesize […]
Active Microemulsification as a Principle of Chromatin Organization and its Role in Cell Fate Induction
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Active Microemulsification as a Principle of Chromatin Organization and its Role in Cell Fate Induction
Active Microemulsification as a Principle of Chromatin Organization and its Role in Cell Fate Induction
The cell nucleus displays a striking, widely conserved spatial compartmentalization. Inactive regions of the genome are sequestered to and compacted within a distinct comparment; active regions of the genome are […]
Molecular Mechanisms of Functionally Distinct Liquid-Liquid Phase Separation of Drosophila Loqs, Ago2 and dsRNA
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Molecular Mechanisms of Functionally Distinct Liquid-Liquid Phase Separation of Drosophila Loqs, Ago2 and dsRNA
Molecular Mechanisms of Functionally Distinct Liquid-Liquid Phase Separation of Drosophila Loqs, Ago2 and dsRNA
Small RNA silencing serves to regulate genes but also as a defense against pathogens and selfish genetic elements. In insects, a specialization of small RNAs and their biogenesis factors for […]
Conformational Selection in Liquid-Liquid Phase Separation: Integrating Protein Folding, Misfolding and Stress Granule Formation
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Conformational Selection in Liquid-Liquid Phase Separation: Integrating Protein Folding, Misfolding and Stress Granule Formation
Conformational Selection in Liquid-Liquid Phase Separation: Integrating Protein Folding, Misfolding and Stress Granule Formation
Intracellular membraneless organelles (MOs) form dynamically and thermodynamically reversible via liquid-liquid phase separation (LLPS). The molecular processes that drive MO formation are largely unknown, but recent deletion experiments showed that […]
Driving Forces of TDP-43 Phase Transitions and their Physiological and Pathological Consequences in Cells
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Driving Forces of TDP-43 Phase Transitions and their Physiological and Pathological Consequences in Cells
Driving Forces of TDP-43 Phase Transitions and their Physiological and Pathological Consequences in Cells
Aberrant liquid-to-solid phase transitions of RNA-binding proteins (RBPs) with prion-like low complexity domains (LCD) are thought to underlie the formation of pathological RBP aggregates in a number of neurodegenerative diseases, […]
Phase Separation in PML Nuclear Bodies through a Multivalent PolySUMO-2/3 Network is required for Efficient DNA Recombination and Repair
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Phase Separation in PML Nuclear Bodies through a Multivalent PolySUMO-2/3 Network is required for Efficient DNA Recombination and Repair
Phase Separation in PML Nuclear Bodies through a Multivalent PolySUMO-2/3 Network is required for Efficient DNA Recombination and Repair
Membrane-less organelles in the nucleus of mammalian cells have been suggested recently to assemble and function via LLPS mechanisms. One main driver of LLPS at PML NBs appears to be […]
Phase Separation of Ki-67 during Mitotic Exit
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Phase Separation of Ki-67 during Mitotic Exit
Phase Separation of Ki-67 during Mitotic Exit
Recent studies have demonstrated that membrane-less condensates assemble by liquid-liquid phase separation of proteins that share multivalency, intrinsic disorder and low complexity sequences as common characteristics. The details of the […]
Molecular mechanisms of functional phase separation in eukaryotic gene transcription
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Molecular mechanisms of functional phase separation in eukaryotic gene transcription
Molecular mechanisms of functional phase separation in eukaryotic gene transcription
Transcription of protein-coding genes by RNA polymerase II (Pol II) is highly regulated during cell differentiation and organismal development. In human cells, Pol II transcription is regulated by ~1,600 transcription […]
The Formation of Phase-Separated Transcription Hubs and their Function in Transcription Regulation
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The Formation of Phase-Separated Transcription Hubs and their Function in Transcription Regulation
The Formation of Phase-Separated Transcription Hubs and their Function in Transcription Regulation
Transcription hubs of transcription factors and RNA polymerase II have been observed in tissue culture cells and embryos. These transcription hubs are assembled through a phase separation mechanism driven by […]
Molecular and Cellular Determinants of Tau Protein Condensation into Dense Liquid Phases
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Molecular and Cellular Determinants of Tau Protein Condensation into Dense Liquid Phases
Molecular and Cellular Determinants of Tau Protein Condensation into Dense Liquid Phases
The neuronal tau protein is involved in the regulation of the axonal microtubule skeleton in the brain. In neurodegenerative diseases such as Alzheimer’s disease (AD) and Frontotemporal dementia (FTD), tau […]
Development of a Combined Fluorescence, Optical Diffraction Tomography and Brillouin (FOB) Microscope for the Quantitative Investigation of Phase Transitions in Cells
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Development of a Combined Fluorescence, Optical Diffraction Tomography and Brillouin (FOB) Microscope for the Quantitative Investigation of Phase Transitions in Cells
Development of a Combined Fluorescence, Optical Diffraction Tomography and Brillouin (FOB) Microscope for the Quantitative Investigation of Phase Transitions in Cells
This project led by the Alberti and Guck teams will develop a new combined fluorescence, optical diffraction tomography and Brillouin (FOB) microscope and to use FOB microscopy to study physiological […]
Molecular mechanisms of physiological and aberrant phase transitions
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Molecular mechanisms of physiological and aberrant phase transitions
Molecular mechanisms of physiological and aberrant phase transitions
The Alberti and Seidel teams will join forces to investigate the conformational dynamics of Fused in Sarcom (FUS) in vitro and in living cells using cutting-edge single molecule technology such […]

SPP2191 of the DFG