Publications

2022

Daniel Schraivogel, Terra M. Kuhn, Benedikt Rauscher, Marta Rodríguez-Martínez, Malte Paulsen, Keegan Owsley, Aaron Middlebrook, Christian Tischer, Beáta Ramasz, Diana Ordoñez-Rueda, Martina Dees, Sara Cuylen-Haering, Eric Diebold, Lars M. Steinmetz (2022) High-speed fluorescence image-enabled cell sorting, Science 375(6578), p. 315-320, American Association for the Advancement of Science, url, doi:10.1126/SCIENCE.ABJ3013/SUPPL_FILE/SCIENCE.ABJ3013_MDAR_REPRODUCIBILITY_CHECKLIST.PDF

Raimund Schlüßler, Kyoohyun Kim, Martin Nötzel, Anna Taubenberger, Shada Abuhattum, Timon Beck, Paul Müller, Shovamayee Maharana, Gheorghe Cojoc, Salvatore Girardo, Andreas Hermann, Simon Alberti, Jochen Guck (2022) Correlative all-optical quantification of mass density and mechanics of subcellular compartments with fluorescence specificity, eLife 11, p. 68490, eLife Sciences Publications, Ltd, url, doi:10.7554/ELIFE.68490

Janine Hochmair, Christian Exner, Maximilian Franck, Alvaro Dominguez-Baquero, Lisa Diez, H Evila Brognaro, Matthew L Kraushar, Thorsten Mielke, Helena Radbruch, Senthil Kaniyappan, Sven Falke, Eckhard Mandelkow, Christian Betzel, Susanne Wegmann (2022) Molecular crowding and RNA synergize to promote phase separation, microtubule interaction, and seeding of Tau condensates, The EMBO Journal, p. e108882, John Wiley & Sons, Ltd, url, doi:10.15252/EMBJ.2021108882

LA Gruijs da Silva, D Dormann (2022) Sedimentation assays to assess the impact of post-translational modifications on phase separation of RNA-binding proteins in vitro and in cells, Methods Mol Biol, in press

LA Gruijs da Silva, F Simonetti, S Hutten, H Riemenschneider, EL Sternburg, LM Pietrek, J Gebel, V Doetsch, D Edbauer, G Hummer, LS Stelzl, D Dormann (2022) Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation, EMBO J, in press

Erin L. Sternburg, Lara A. Gruijs da Silva, Dorothee Dormann (2022) Post-translational modifications on RNA-binding proteins: accelerators, brakes, or passengers in neurodegeneration?, Trends in biochemical sciences 47(1), p. 6-22, Trends Biochem Sci, url, doi:10.1016/J.TIBS.2021.07.004

2021

Nirnay Samanta, Sara S. Ribeiro, Mailin Becker, Emeline Laborie, Roland Pollak, Stepan Timr, Fabio Sterpone, Simon Ebbinghaus (2021) Sequestration of Proteins in Stress Granules Relies on the In-Cell but Not the in Vitro Folding Stability, Journal of the American Chemical Society 143(47), p. 19909-19918, American Chemical Society, url, doi:10.1021/JACS.1C09589/SUPPL_FILE/JA1C09589_SI_001.PDF

Hamideh Hajiabadi, Irina Mamontova, Roshan Prizak, Agnieszka Pancholi, Anne Koziolek, Lennart Hilbert (2021) Deep-learning microscopy image reconstruction with quality control reveals second-scale rearrangements in RNA polymerase II clusters, bioRxiv, p. 2021.12.05.471272, Cold Spring Harbor Laboratory, url, doi:10.1101/2021.12.05.471272

Giacomo Bartolucci, Omar Adame-Arana, Xueping Zhao, Christoph A. Weber (2021) Controlling composition of coexisting phases via molecular transitions, Biophysical Journal 120(21), p. 4682-4697, Elsevier, url, doi:10.1016/j.bpj.2021.09.036

Xueping Zhao, Giacomo Bartolucci, Alf Honigmann, Frank Jülicher, Christoph A Weber (2021) Thermodynamics of wetting, prewetting and surface phase transitions with surface binding, New Journal of Physics 23(12), p. 123003, IOP Publishing, url, doi:10.1088/1367-2630/ac320b

Lars Hubatsch, Louise M. Jawerth, Celina Love, Jonathan Bauermann, T. Y.Dora Tang, Stefano Bo, Anthony A. Hyman, Christoph A. Weber (2021) Quantitative theory for the diffusive dynamics of liquid condensates, eLife 10, eLife Sciences Publications Ltd, doi:10.7554/eLife.68620

Salma Sohrabi-Jahromi, Johannes Söding (2021) Thermodynamic modeling reveals widespread multivalent binding by RNA-binding proteins, Bioinformatics 37(Supplement_1), p. I308-I316, Oxford Academic, url, doi:10.1093/bioinformatics/btab300

Chad A. Sallaberry, Barbie J. Voss, Jaroslaw Majewski, Jacek Biernat, Eckhard Mandelkow, Eva Y. Chi, Crystal M. Vander Zanden (2021) Tau and Membranes: Interactions That Promote Folding and Condensation, Frontiers in Cell and Developmental Biology 9, Front Cell Dev Biol, url, doi:10.3389/fcell.2021.725241

Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter (2021) Disease-Associated Tau Phosphorylation Hinders Tubulin Assembly within Tau Condensates, Angewandte Chemie - International Edition 60(2), p. 726-730, John Wiley & Sons, Ltd, url, doi:10.1002/anie.202011157

Susanne Wegmann, Jacek Biernat, Eckhard Mandelkow (2021) A current view on Tau protein phosphorylation in Alzheimer's disease, Current Opinion in Neurobiology 69, p. 131-138, Curr Opin Neurobiol, url, doi:10.1016/j.conb.2021.03.003

Prashant Rawat, Marc Boehning, Barbara Hummel, Fernando Aprile-Garcia, Anwit S. Pandit, Nathalie Eisenhardt, Ashkan Khavaran, Einari Niskanen, Seychelle M. Vos, Jorma J. Palvimo, Andrea Pichler, Patrick Cramer, Ritwick Sawarkar (2021) Stress-induced nuclear condensation of NELF drives transcriptional downregulation, Molecular Cell 81(5), p. 1013-1026.e11, Mol Cell, url, doi:10.1016/j.molcel.2021.01.016

Jasper J. Michels, Mateusz Brzezinski, Tom Scheidt, Edward A. Lemke, Sapun H. Parekh (2021) Role of Solvent Compatibility in the Phase Behavior of Binary Solutions of Weakly Associating Multivalent Polymers, Biomacromolecules 15, p. acs.biomac.1c01301, American Chemical Society, url, doi:10.1021/ACS.BIOMAC.1C01301

Sayantan Chatterjee, Yelena Kan, Mateusz Brzezinski, Kaloian Koynov, Roshan Mammen Regy, Anastasia C. Murthy, Kathleen A. Burke, Jasper J. Michels, Jeetain Mittal, Nicolas L. Fawzi, Sapun H. Parekh (2021) Reversible Kinetic Trapping of FUS Biomolecular Condensates, Advanced Science, p. 2104247, John Wiley & Sons, Ltd, url, doi:10.1002/ADVS.202104247

Lukas Frank, Robin Weinmann, Fabian Erdel, Jorge Trojanowski, Karsten Rippe (2021) Transcriptional Activation of Heterochromatin by Recruitment of dCas9 Activators, Enhancer and Promotors. Methods in Molecular Biology 2351, T. Borggrefe, B.D. Giaimo (ed.), p. 307-320, Humana, New York, NY, url, doi:10.1007/978-1-0716-1597-3_17

Jorge Trojanowski, Lukas Frank, Anne Rademacher, Pranas Grigaitis, Karsten Rippe (2021) Transcription activation is enhanced by multivalent interactions independent of liquid-liquid phase separation, bioRxiv, p. 2021.01.27.428421, Cold Spring Harbor Laboratory, url, doi:10.1101/2021.01.27.428421

Amra Noa, Hui Shun Kuan, Vera Aschmann, Vasily Zaburdaev, Lennart Hilbert (2021) The hierarchical packing of euchromatin domains can be described as multiplicative cascades, PLoS Computational Biology 17(5), p. e1008974, Public Library of Science, url, doi:10.1371/journal.pcbi.1008974

Agnieszka Pancholi, Tim Klingberg, Weichun Zhang, Roshan Prizak, Irina Mamontova, Amra Noa, A.Y. Kobitski, G.U. Nienhaus, Vasily Zaburdaev, Lennart Hilbert (2021) RNA polymerase II clusters form in line with liquid phase wetting of chromatin, bioRxiv, p. 2021.02.03.429626, Cold Spring Harbor Laboratory, url, doi:10.1101/2021.02.03.429626

Bart W. Hoogenboom, Loren E. Hough, Edward A. Lemke, Roderick Y.H. Lim, Patrick R. Onck, Anton Zilman (2021) Physics of the nuclear pore complex: Theory, modeling and experiment, Physics Reports 921, p. 1-53, North-Holland, doi:10.1016/j.physrep.2021.03.003

Amra Noa, Hui Shun Kuan, Vera Aschmann, Vasily Zaburdaev, Lennart Hilbert (2021) The hierarchical packing of euchromatin domains can be described as multiplicative cascades, PLoS Computational Biology 17(5), p. e1008974, Public Library of Science, url, doi:10.1371/journal.pcbi.1008974

Lara Aletta Gruijs da Silva, Francesca Simonetti, Saskia Hutten, Henrick Riemenschneider, Erin L Sternburg, Lisa M Pietrek, Jakob Gebel, Volker Doetsch, Dieter Edbauer, Gerhard Hummer, Lukas S Stelzl, Dorothee Dormann (2021) Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation, bioRxiv, p. 2021.04.30.442163, Cold Spring Harbor Laboratory, url, doi:10.1101/2021.04.30.442163

2020

A. Klosin, F. Oltsch, T. Harmon, A. Honigmann, F. Jülicher, A. A. Hyman, C. Zechner (2020) Phase separation provides a mechanism to reduce noise in cells, Science 367(6476), p. 464-468, American Association for the Advancement of Science, url, doi:10.1126/SCIENCE.AAV6691/SUPPL_FILE/AAV6691S2.MOV

Anne Bremer, Tanja Mittag, Michael Heymann (2020) Microfluidic characterization of macromolecular liquid-liquid phase separation, Lab on a Chip 20(22), p. 4225-4234, The Royal Society of Chemistry, url, doi:10.1039/d0lc00613k

Sara Cuylen-Haering, Mina Petrovic, Alberto Hernandez-Armendariz, Maximilian W.G. Schneider, Matthias Samwer, Claudia Blaukopf, Liam J. Holt, Daniel W. Gerlich (2020) Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly, Nature 2020 587:7833 587(7833), p. 285-290, Nature Publishing Group, url, doi:10.1038/s41586-020-2672-3

Johannes Söding, David Zwicker, Salma Sohrabi-Jahromi, Marc Boehning, Jan Kirschbaum (2020) Mechanisms for Active Regulation of Biomolecular Condensates, Trends in Cell Biology 30(1), p. 4-14, Elsevier, url, doi:10.1016/j.tcb.2019.10.006

Tina Ukmar-Godec, Susanne Wegmann, Markus Zweckstetter (2020) Biomolecular condensation of the microtubule-associated protein tau, Seminars in Cell & Developmental Biology 99, p. 202-214, Academic Press, doi:10.1016/J.SEMCDB.2019.06.007

Giorgia Celetti, Giulia Paci, Joana Caria, Virginia VanDelinder, George Bachand, Edward A. Lemke (2020) The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes, Journal of Cell Biology 219(1), Rockefeller University Press, url, doi:10.1083/jcb.201907157

Jaroslaw Majewski, Emmalee M. Jones, Crystal M. Vander Zanden, Jacek Biernat, Eckhard Mandelkow, Eva Y. Chi (2020) Lipid membrane templated misfolding and self-assembly of intrinsically disordered tau protein, Scientific Reports 10(1), p. 1-13, Nature Publishing Group, url, doi:10.1038/s41598-020-70208-6

Ariel Erijman, Lukasz Kozlowski, Salma Sohrabi-Jahromi, James Fishburn, Linda Warfield, Jacob Schreiber, William S. Noble, Johannes Söding, Steven Hahn (2020) A High-Throughput Screen for Transcription Activation Domains Reveals Their Sequence Features and Permits Prediction by Deep Learning, Molecular Cell 78(5), p. 890-902.e6, Cell Press, doi:10.1016/j.molcel.2020.04.020

Nike Heinß, Mikhail Sushkin, Miao Yu, Edward A. Lemke (2020) Multifunctionality of F-rich nucleoporins, Biochemical Society Transactions 48(6), p. 2603-2614, Portland Press, url, doi:10.1042/BST20200357

Lukas Frank, Karsten Rippe (2020) Repetitive RNAs as Regulators of Chromatin-Associated Subcompartment Formation by Phase Separation, Journal of Molecular Biology 432(15), p. 4270-4286, Academic Press, doi:10.1016/j.jmb.2020.04.015

Carsten Donau, Fabian Späth, Marilyne Sosson, Brigitte A.K. Kriebisch, Fabian Schnitter, Marta Tena-Solsona, Hyun Seo Kang, Elia Salibi, Michael Sattler, Hannes Mutschler, Job Boekhoven (2020) Active coacervate droplets as a model for membraneless organelles and protocells, Nature Communications 11(1), p. 1-10, Nature Publishing Group, url, doi:10.1038/s41467-020-18815-9

Saskia Hutten, Dorothee Dormann (2020) A Quantitative Assay to Measure Stress Granule Association of Proteins and Peptides in Semi-permeabilized Human Cells, BIO-PROTOCOL 10(24), p. e3846, Bio Protoc, url, doi:10.21769/bioprotoc.3846

2019

Tina Ukmar-Godec, Saskia Hutten, Matthew P. Grieshop, Nasrollah Rezaei-Ghaleh, Maria Sol Cima-Omori, Jacek Biernat, Eckhard Mandelkow, Johannes Söding, Dorothee Dormann, Markus Zweckstetter (2019) Lysine/RNA-interactions drive and regulate biomolecular condensation, Nature Communications 10(1), p. 1-15, Nature Publishing Group, url, doi:10.1038/s41467-019-10792-y

Sara S. Ribeiro, Nirnay Samanta, Simon Ebbinghaus, João C. Marcos (2019) The synergic effect of water and biomolecules in intracellular phase separation, Nature Reviews Chemistry 3(9), p. 552-561, Nature Publishing Group, url, doi:10.1038/s41570-019-0120-4