Alberti/Seidel

Molecular mechanisms of physiological and aberrant phase transitions

The Alberti and Seidel teams will join forces to investigate the conformational dynamics of Fused in Sarcom (FUS) in vitro and in living cells using cutting-edge single molecule technology such as Förster energy transfer (FRET), fluorescence correlation spectroscopy (FCS) and integrative FRET-restrained structural modelling. We aim to characterize the conformational dynamics of FUS in the four different material states in vitro (soluble, liquid droplet, gel, fiber). We will then determine how the structure and dynamics of FUS is affected by RNA and protein binding partners. Next, we will compare the conformational dynamics of FUS in vitro with those in vivo in the nucleus, cytoplasm, and in physiological and aberrant condensates. Finally, we will generate FUS variants with specific defects in phase separation behavior and test these variants in living cells to gain insight into physiology and disease mechanisms. In summary, we aim to uncover the molecular events underlying the formation of cellular condensates, thus generating a detailed quantitative understanding of the molecular mechanisms driving phase separation in living cells.


About us

Contact:
Simon Alberti
Technische Universität Dresden
Biotechnology Center
Tatzberg 47/49
01307 Dresden
Email
Phone: +49 (0)351 463 40236

 

 

Contact:
Claus Seidel
Heinrich Heine University
Chair of Molecular Physical Chemistry
Universitätsstr. 1, Building 26.32.02, room 44
40225 Düsseldorf
Email
Phone: +49 211 81-15881